Abstract:
The aim of this study was to investigate the effect of etodolac hydrazone (EH),
a new compound synthesised from etodolac, on spermatozoon quality, testicular
lipid peroxidation, apoptosis and spermatozoon DNA integrity in rats. Group 1
(n = 8) received 1 ml dimethyl sulfoxide (DMSO) daily (Control); group 2
(n = 8) was treated with 5 mg kg
1 day
1 EH, dissolved in 1 ml DMSO (EH-
5); and group 3 (n = 8) was treated with 10 mg kg
1 day
1 EH, dissolved in
1 ml DMSO (EH-10). All administrations were performed by gavage and maintained
for 8 weeks. Both doses of EH administration caused significant
decreases in absolute and relative weights of testis, whole epididymis, right cauda
epididymis, and spermatozoon motility, spermatozoon count in comparison
with the control group. Only 10 mg kg
1 day
1 EH administration caused significant
decreases in absolute and relative weights of seminal vesicles and serum
testosterone level, and significant increases in testicular lipid peroxidation level,
and numbers of TUNEL+ apoptotic germ cells and spermatozoa with damaged
DNA along with some histopathological damages when compared to the control
group. However, body and ventral prostate weight, and testicular antioxidant
markers (glutathione, glutathione-peroxidase and catalase), were unaffected significantly
by both doses of EH administration. In conclusion, two different
doses of EH, in particular its high dose, damage to testicular spermatogenic cells
and spermatozoon DNA and, it decreases spermatozoon motility, count and testosterone
level in healthy rats.
