Abstract:
This study was conducted to investigate the effects of
intratesticular glycerol and ethanol injection on
sterilization.
Twelve mature male dogs were used. They were
allocated randomly to two equal groups called glycerol
and ethanol and one ml glycerol (70%) and ethanol
(95%) was injected intratesticularly. The animals were
observed during two months. The blood and sperm
samples obtained once a week in all groups were
examined regarding to serum testosterone level and
spermatozoon concentration and motility rate. There was
a fistula formation and scrotal wounds resulting from
orchitis in all glycerol and two ethanol group dogs after
the 5th day of injection. The fistular openings closed
spontaneously in all cases in due course. After the 2nd
month, testicular atrophy was seen to occur. It was
determined the presence of marked decreases (p<0.01) in
both groups spermatozoon concentration and motility as
well as in the level of serum testosterone two weeks after
drug injection. The histopathologic examination
conducted after the 2nd month revealed degeneration,
necrosis and diffuse connective tissue proliferation in the
seminiferus contortus tubulus of both groups.
It has been concluded that glycerol and ethanol can be
effective for sterilization in male dogs.
