http://hdl.handle.net/11508/11926 Wed, 29 Apr 2026 11:44:26 GMT 2026-04-29T11:44:26Z http://hdl.handle.net/11508/20951 Evaluation of cardiac troponin I in serum and myocardium of rabbits with experimentally induced polymicrobial sepsis Tümer, Kenan Çağrı; Özdemir, Haydar; Eröksüz, Hatice Sepsis is a potentially life-threatening condition, and it is frequently complicated by myocardial damage. Data on myocardial damage in rabbit caecal ligation and puncture (CLP) models are limited, although numerous animal models have been used to study sepsis-associated myocardial damage. This study aimed to investigate the effect of CLP on cardiac muscle by measuring serum cardiac troponin I (cTnI) concentrations and by detecting both histopathological changes and cTnI immunoreactivity in cardiomyocytes in rabbits. After CLP was performed in rabbits, blood samples were taken from the jugular vein at 0, 4, 8, and 12 h for haematological and biochemical analyses. At the end of the experiment, all of the rabbits were euthanised to examine the histopathological changes and the cTnI immunoreactivity in cardiac muscle tissue. No changes in serum cTnI concentration were observed in the experimental group (EG) or control group (CG) at 0 and 4 h. In EG, the mean serum cTnI concentrations were 0.230 ± 0.209 and 1.177 ± 0.971 ng/ml at 8 and 12 h, respectively. In CG, the mean serum cTnI concentrations were 0.032 ± 0.014 and 0.031 ± 0.021 ng/ml at 8 and 12 h, respectively. Moreover, cytoplasmic cTnI immunoreactivity decreased in EG compared with that in CG (P<0.01). The results demonstrated that CLP induced a systemic inflammatory response and caused myocardial damage in rabbits. Wed, 29 Jan 2020 00:00:00 GMT http://hdl.handle.net/11508/20951 2020-01-29T00:00:00Z http://hdl.handle.net/11508/8911 Farklı tüy rengine sahip Japon bıldırcınlarında (Coturnix coturnix japonica) yumurtanın besin özellikleri, kuluçka özellikleri ve embriyonun beslenmesi Şimşek, Ülkü Gülcihan; Erişir, Zeki; Mutlu, Seda İflazoğlu; Baykalır, Yasin; Çiftçi, Mehmet Bu araştırma gri ve sarı tüy rengine sahip Japon bıldırcını gruplarında bazı yumurta kalite özellikleri, besin kompozisyonu, kuluçka özellikleri ve embriyonun beslenmesini incelemek amacıyla yürütülmüştür. Bu amaç için her çalışma grubundan 240 adet olacak şekilde toplam 480 adet yumurta kullanılmıştır. Araştırmada her iki grupta ağırlıkları eşitlenerek değerlendirilmeye alınan taze yumurtaların ak, sarı ve kabuk ağlıkları ile oranları benzer bulunmuştur (P>0.05). Yumurtanın besin özelliklerinden kuru madde, kül, ham protein ve ham yağ değerlerinde gruplar arasında istatistiki farklılık saptanmamıştır (P>0.05). Kuluçkanın 15. gününde incelenen yumurta gruplarında ağırlık kaybı, embriyo ağırlığı, sarı kesesi ağırlığı, kabuk ağırlığı, kabukta ham kül değeri, sarı kesesi kuru madde, ham protein, ham yağ, ham kül değerine ait ortalamalarda gruplar birbirine benzer olduğu tespit edilmiştir (P>0.05). On beş günlük kuluçka süresince embriyo yumurta akının tamamını kullanmış, kabuk ham kül oranı %1.42, sarı kesesi ham kül oranı %10.42 azalmıştır. Embriyo yumurta sarı ham proteininin %38.87’sini, yağın %8.71’ini kullanmıştır. Kuluçka özelliklerinden döllülük oranı, kuluçka randımanı, çıkım gücü ve toplam embriyo ölüm oranı bakımından gruplar benzerdir (P>0.05). Yumurtanın civcive dönüşüm oranında gri renkli grubun rakamsal üstünlüğü önemli bulunmamıştır (P=0.063). Sonuç olarak yumurta ve kuluçka özellikleri bakımından gri veya sarı tüylü Japon bıldırcınlarında gruplar benzerlik gösterdiği tespit edilmiştir.; This study was conducted to investigate some quality traits, nutrient composition, hatchability, and nutrition of embryos in eggs of Japanese quails with gray and golden plumage colors. For this purpose, a total of 480 eggs, 240 eggs per experimental group were used. Weights or percentages of albumen, yolk and shell were found to be similar in fresh eggs which have equalized egg weight in two experimental groups of the research (P>0.05). There was no statistical difference in dry matter, ash, crude protein and crude fat values related to nutritional traits of eggs between the groups (P>0.05). Weight loss, embryo weight, yolk sac weight, shell weight, crude ash value of shell, yolk sac dry matter, crude protein, crude fat, crude ash values were found similar between two groups in evaluated eggs at the 15th day of hatching period (P>0.05). During the fifteenth day of incubation, albumen was consumed completely by embryos, crude ash value of shell and yolk sac were decreased 1.42% and 10.42%, respectively. The embryo consumed 38.87% of crude protein and 8.71% of crude fat of egg yolk. The fertility, hatchability, hatchability of fertile eggs and total embryonic mortality rate were similar between groups (P>0.05). Numerical superiority of gray group at conversion ratio of egg to chick was not significant (P=0.063). Consequently, Japanese quail groups have gray or golden plumage were similar in terms of egg and hatchability traits. Fri, 01 Jan 2016 00:00:00 GMT http://hdl.handle.net/11508/8911 2016-01-01T00:00:00Z http://hdl.handle.net/11508/8877 Comparative evaluation of the sexual functions and NF-κB and Nrf2 pathways of some aphrodisiac herbal extracts in male rats Şahin, Kazım; Orhan, Cemal; Akdemir, Fatih; Tuzcu, Mehmet; Gençoğlu, Hasan; Şahin, Nurhan; Türk, Gaffari; Yılmaz, İsmet; Özercan, İbrahim H.; Juturu, Vijaya Background: Mucuna pruriens, Tribulus terrestris and Ashwagandha (Withania somnifera) are widely known as antioxidant effective herbals and have been reported to possess aphrodisiac activities in traditional usages. In this study, we determined the effects of these herbals on sexual functions, serum biochemical parameters, oxidative stress and levels of NF-κB, Nrf2, and HO-1 in reproductive tissues. Methods: Thirty-five male rats were divided into five groups: the control group, sildenafil-treated group (5 mg/kg/d), Mucuna, Tribulus and Ashwagandha groups. The extract groups were treated orally either with Mucuna, Tribulus or Ashwagandha (300 mg/kg b.w.) for 8 weeks. Results: All of the extracts were found to be significantly effective in sexual functioning and antioxidant capacity and Tribulus showed the highest effectiveness. Serum testosterone levels significantly increased in Tribulus and Ashwagandha groups in comparison to control group. Tribulus was able to reduce the levels of NF-κB and increase the levels of Nrf2 and HO-1 to a much greater extent than Mucuna and Ashwagandha. Conclusions: These results demonstrate for the first time that Mucuna, Tribulus and Ashwagandha supplementation improves sexual function in male rats via activating Nrf2/ HO-1 pathway while inhibiting the NF-κB levels. Moreover, Tribulus terrestris extract was found to be more bioavailable from Ashwagandha extract followed by Mucuna extract. Fri, 01 Jan 2016 00:00:00 GMT http://hdl.handle.net/11508/8877 2016-01-01T00:00:00Z http://hdl.handle.net/11508/8795 Effects of cinnamon (C. zeylanicum) bark oil against taxanes-induced damages in sperm quality, testicular and epididymal oxidant/antioxidant balance, testicular apoptosis, and sperm DNA integrity Sarıözkan, Serpil; Türk, Gaffari; Güvenç, Mehmet; Yüce, Abdurrauf; Özdamar, Saim; Cantürk, Fazile; Yay, Arzu Hanım The aim of this study was to investigate whether cinnamon bark oil (CBO) has protective effect on taxanes-induced adverse changes in sperm quality, testicular and epididymal oxidant/antioxidant balance, testicular apoptosis, and sperm DNA integrity. For this purpose, 88 adult male rats were equally divided into 8 groups: control, CBO, docetaxel (DTX), paclitaxel (PTX), DTX+PTX, DTX+CBO, PTX+CBO, and DTX+PTX+CBO. CBO was given by gavage daily for 10 weeks at the dose of 100 mg/kg. DTX and PTX were administered by intraperitoneal injection at the doses of 5 and 4 mg/ kg/week, respectively, for 10 weeks. DTX+PTX and DTX+PTX+CBO groups were treated with DTX during first 5 weeks and PTX during next 5 weeks. DTX, PTX, and their mixed administrations caused significant decreases in absolute and relative weights of all reproductive organs, testosterone level, sperm motility, concentration, glutathione level, and catalase activity in testicular and epididymal tissues. They also significantly increased abnormal sperm rate, testicular and epididymal malondialdehyde level, apoptotic germ cell number, and sperm DNA fragmentation and significantly damaged the histological structure of testes. CBO consumption by DTX-, PTX-, and DTX+PTX-treated rats provided significant ameliorations in decreased relative weights of reproductive organs, decreased testosterone, decreased sperm quality, imbalanced oxidant/ antioxidant system, increased apoptotic germ cell number, rate of sperm with fragmented DNA, and severity of testicular histopathological lesions induced by taxanes. In conclusion, taxanes cause impairments in sperm quality, testicular and epididymal oxidant/antioxidant balance, testicular histopathological structure, and sperm DNA integrity, and long-term CBO consumption protects male reproductive system of rats. Wed, 23 Mar 2016 00:00:00 GMT http://hdl.handle.net/11508/8795 2016-03-23T00:00:00Z